Cloned (Comment) | Organism |
---|---|
heterologously overproduced in Escherichia coli | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
5-dehydro-D-fructose | pH and temperature not specified in the publication | Clostridium pasteurianum | |
12.4 | - |
NADPH | pH and temperature not specified in the publication | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35900 | - |
molecular mass including Strep-tag | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-sorbose + NADP+ | Clostridium pasteurianum | - |
5-dehydro-D-fructose + NADPH + H+ | - |
? | |
L-sorbose + NADP+ | Clostridium pasteurianum DSM 525 | - |
5-dehydro-D-fructose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | AJA48273.1 | - |
- |
Clostridium pasteurianum DSM 525 | AJA48273.1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.9 | - |
pH and temperature not specified in the publication | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-1,2-propandione + NADP+ | 14.8% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum | ? | - |
? | |
1-phenyl-1,2-propandione + NADP+ | 14.8% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum DSM 525 | ? | - |
? | |
5-dehydro-D-fructose + NADPH + H+ | - |
Clostridium pasteurianum | L-sorbose + NADP+ | - |
? | |
5-dehydro-D-fructose + NADPH + H+ | - |
Clostridium pasteurianum DSM 525 | L-sorbose + NADP+ | - |
? | |
diacetyl + NADP+ | 38% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum | ? | - |
? | |
diacetyl + NADP+ | 38% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum DSM 525 | ? | - |
? | |
L-sorbose + NADP+ | - |
Clostridium pasteurianum | 5-dehydro-D-fructose + NADPH + H+ | - |
? | |
L-sorbose + NADP+ | - |
Clostridium pasteurianum DSM 525 | 5-dehydro-D-fructose + NADPH + H+ | - |
? | |
pyruvaldehyde + NADP+ | 5% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum | ? | - |
? | |
pyruvaldehyde + NADP+ | 5% of the specific activity as compared to 5-dehydro-D-fructose | Clostridium pasteurianum DSM 525 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Clostridium pasteurianum |
Synonyms | Comment | Organism |
---|---|---|
AJA48273.1 | - |
Clostridium pasteurianum |
CP-AKR | - |
Clostridium pasteurianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Clostridium pasteurianum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.3 | - |
5-dehydro-D-fructose | pH and temperature not specified in the publication | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Clostridium pasteurianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Clostridium pasteurianum |
General Information | Comment | Organism |
---|---|---|
metabolism | an important function in the degradation of this substrate and might be responsible for the formation of fructose, which is then channelled into the central metabolism of Clostridium pasteurianum | Clostridium pasteurianum |